Biophysium

Metabolic diseases

Metabolic diseases

Metabolic diseases are related to disorders of cellular metabolism. The most common non-hereditary and lifestyle-related metabolic disorders are obesity, diabetes, hypertension, hyperlipidemia, hypercholesterolemia, hypertriglyceridemia, acute hyperglycemia, NASH, metabolic syndrome or gout. These frequent disorders can be at the origin of cardiovascular, neurodegenerative or cancerous pathologies, thus constituting a major public health issue.

Biophysium provides you many validated preclinical and clinical models as well as a large expertise in physiology with functional explorations, metabolic and proteomic analyses in order to test the health effect of your drug candidate or your natural product as well as its mode of administration.

In order to meet your requirements, we are able to use different rodent models obtained by pharmacological interventions, diet modifications but also genetically modified models. The recording of basic physiological parameters (weight, food intake, behavior) is common to all our animal models.

maladie
Pre-clinical models
rongeur

Hypertension 

In vivo or ex vivo myocardial ischemia

Heart failure

Dyslipidemia and Atherosclerosis

 ApoE-/- or KO mice for LDL receptor

Obesity

High Fat diet, ob/ob rat and db/db mouse

Type 1 or 2 Diabetes

Zucker or Goto-Kakizaki rat, type 1 – streptozotocine

Acute hyperglycemia

Hypercholesterolemia

Hyperlipidemia

in vivo  and in vitro digestion models

Customized diet adapted to your project

Voluntary or imposed exercise

Wheel or treadmill

Western diet

Sweetener supplementation

Isolated cardiomyocyte

Oxidative stress production

Mitochondrial function

Calcium retention capacity, fusion, fission, swelling, oxidative stress

Isolated models of heart and vessels

Bioavailability, metabolism and biodistribution of compounds

HPLC -MS-MS analysis for specific biomarkers

Compound administration

Injection, gavage, drinking water, food

Samples: urine, faeces, blood or tissue/organ

Clinical models

Obese

Diabetic

Cardiovascular risk factors

Voluntary or imposed physical exercise

Microcirculatory function

Technical platform and transversal analyses

Biochemical tests: Western blot, ELISA kit, protein activity, S-nitrosoproteomic

Glucose homeostasis: fasting blood glucose measurement, glucose tolerance test, insulin tolerance test

Lipid tolerance test

Lipid metabolism and oxidation

Adipose tissue analysis

 Evaluation of hepatic lipids, steatosis and fibrosis

Blood pressure measurement by non-invasive plethysmography (tail cuff method)

Measurement of atheromatous plaque size by ultrasound, histology and colorimetry

Isolated vessel model and vasoreactivity test

Aorta, coronary, pulmonary

Langendorff perfused isolated heart model

Electrocardiogram

Endothelial function

Body composition and adiposity index

HOMA-IR index

Blood lipid profile and oxidation 

Telemetry monitoring

Metabolomic and proteomic

Oxidative stress, inflammation, fibrosis in different organs/tissues

S-Nitrosylation, cytosolic and mitochondrial regulation of oxidative stress production

Hormones

Insulin, glucagon, adiponectin, leptin, etc..

Inflammatory cytokines 

Interleukine 1b, 6, MCP-1, TNF-alpha, etc..

VCAM-1 et ICAM-1

Histology

macrophage infiltration, remodeling, staining: hematoxylin-eosin, Sirius Red, Oil-Red-O, Masson Trichome, PAS staining

Immunohistology on resquest

Gene expression: lipogenesis, lipolysis, carbohydrate metabolism 

Samples: urine, faeces, blood or organ/tissue

Cardiac and vascular imaging by ultrasound:
systolic and diastolic function, regional myocardial deformation, cardiac and vascular morphology

HPLC -MS/MS analysis for specific biomarkers

Complete blood analysis

Calcium transient and contractility

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