Biophysium
Metabolic diseasesMetabolic diseases
Metabolic diseases are related to disorders of cellular metabolism. The most common non-hereditary and lifestyle-related metabolic disorders are obesity, diabetes, hypertension, hyperlipidemia, hypercholesterolemia, hypertriglyceridemia, acute hyperglycemia, NASH, metabolic syndrome or gout. These frequent disorders can be at the origin of cardiovascular, neurodegenerative or cancerous pathologies, thus constituting a major public health issue.
Biophysium provides you many validated preclinical and clinical models as well as a large expertise in physiology with functional explorations, metabolic and proteomic analyses in order to test the health effect of your drug candidate or your natural product as well as its mode of administration.
In order to meet your requirements, we are able to use different rodent models obtained by pharmacological interventions, diet modifications but also genetically modified models. The recording of basic physiological parameters (weight, food intake, behavior) is common to all our animal models.
Pre-clinical models
Hypertension
In vivo or ex vivo myocardial ischemia
Heart failure
Dyslipidemia and Atherosclerosis
ApoE-/- or KO mice for LDL receptor
Obesity
High Fat diet, ob/ob rat and db/db mouse
Type 1 or 2 Diabetes
Zucker or Goto-Kakizaki rat, type 1 – streptozotocine
Acute hyperglycemia
Hypercholesterolemia
Hyperlipidemia
in vivo and in vitro digestion models
Customized diet adapted to your project
Voluntary or imposed exercise
Wheel or treadmill
Western diet
Sweetener supplementation
Isolated cardiomyocyte
Oxidative stress production
Mitochondrial function
Calcium retention capacity, fusion, fission, swelling, oxidative stress
Isolated models of heart and vessels
Bioavailability, metabolism and biodistribution of compounds
HPLC -MS-MS analysis for specific biomarkers
Compound administration
Injection, gavage, drinking water, food
Samples: urine, faeces, blood or tissue/organ
Clinical models
Overweight/Obese
Diabetic
Cardiovascular risk factors
Voluntary or imposed physical exercise
Microcirculatory function
Technical platform and transversal analyses
Biochemical tests: Western blot, ELISA kit, protein activity, S-nitrosoproteomic
Glucose homeostasis: fasting blood glucose measurement, glucose tolerance test, insulin tolerance test
Lipid tolerance test
Lipid metabolism and oxidation
Adipose tissue analysis
Evaluation of hepatic lipids, steatosis and fibrosis
Blood pressure measurement by non-invasive plethysmography (tail cuff method)
Measurement of atheromatous plaque size by ultrasound, histology and colorimetry
Isolated vessel model and vasoreactivity test
Aorta, coronary, pulmonary
Langendorff perfused isolated heart model
Electrocardiogram
Endothelial function
Body composition and adiposity index
HOMA-IR index
Blood lipid profile and oxidation
Telemetry monitoring
Metabolomic and proteomic
Oxidative stress, inflammation, fibrosis in different organs/tissues
S-Nitrosylation, cytosolic and mitochondrial regulation of oxidative stress production
Hormones
Insulin, glucagon, adiponectin, leptin, etc..
Inflammatory cytokines
Interleukine 1b, 6, MCP-1, TNF-alpha, etc..
VCAM-1 et ICAM-1
Histology
macrophage infiltration, remodeling, staining: hematoxylin-eosin, Sirius Red, Oil-Red-O, Masson Trichome, PAS staining
Immunohistology on resquest
Gene expression: lipogenesis, lipolysis, carbohydrate metabolism
Samples: urine, faeces, blood or organ/tissue
Cardiac and vascular imaging by ultrasound:
systolic and diastolic function, regional myocardial deformation, cardiac and vascular morphology
HPLC -MS/MS analysis for specific biomarkers
Complete blood analysis
Calcium transient and contractility
Perfusion Flow
Laser spekcle contrast imaging
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